Xenon lamp excitation and Hamamatsu Orca camera. Multiple filter combination capabilities. Inverted microscope with an enclosed temperature, humidity, and CO2 environmental control chamber. Easily images live cell culture using glass bottom coverslip dishes or fixed tissue/fixed cultures. The timelapse/FRET microscope is capable of FRET, long-term multi-point timelapse, and TIRF imaging. Standard brightfield and epifluorescence. The IX70 microscope is capable of observation of cell culture and slides. Software includes measurement, post-processing, and ‘smart montage’ capabilities. Mercury lamp excitation and epifluorescence capabilities for blue to near red emitting fluorophores.
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Full complement of objectives and a DP74 high resolution camera. The BX51 microscope is capable of standard brightfield and epifluorescence microscopy, polarized light microscopy, phase contrast and differential interference microscopy. Not for high resolution work, but excellent for quick checks of staining or expression progress. Best used for checking progress of culture expression or slide staining. The ZOE imaging system allows for quick observation of cell cultures and slides. Below is a brief summary of the capabilities of each microscope.
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Callahan also maintains the microscopes, working with vendors as needed to upgrade or maintain instruments. Linda Callahan trains all users on each of the microscopes and assists with experimental design and trouble-shooting of imaging experiments. The 5 microscopes within the core include a ZOE imaging system (ZOE), an upright epifluorescence microscope (BX51), an inverted epifluorescence microscope (IX 70), a timelapse/FRET microscope (capable of FRET, multipoint timelapse imaging, and TIRF), and a laser point scanning confocal microscope (FV1000 confocal).
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The Microscopy Core works in conjunction with the CVRI Histology core and the microsurgery core for design and interpretation of microscopy-related experiments. The CVRI Microscopy Core consists of 5 well-maintained microscopes. Leica Cryostat CM 1950 Sakura Tissue-Tek TEC 5ĬVRI MICROSCOPY Core: SUMMARY OF CAPABILITIES.According to the goal of projects and the animal models of human diseases, the core also provide customizing assistance to obtain high quality data and the training for CVRI personnel using histology equipment. The histology core's mission is to provide processing, embedding, sectioning and staining of animal or human tissues in a timely manner.